IFNA21 ELISA Kits Search Results


ifn-α human verikinetm elisa kit  (PBL Assay)
90
PBL Assay ifn-α human verikinetm elisa kit
IFN-α, -β, -λ gene expression and protein release in MDM/TNF/IFNg, MDM/IL4 and MDM. MDM/TNF/IFNg, MDM/IL4 and MDM were treated with infectious RV16 or media alone and cells and supernatants were harvested at 8 h and 24 h or 72 h as indicated. Cell mRNA expression of IFN-α subtypes detected by primers pairs IFN-α.1 and IFN-α.2, panels (a) and (b) and mRNA expression of IFNB (c), IFN λ2/3 (d) and IFN λ1 (e) were measured by RT-PCR. Release of IFN-α (f), -β (g), -λ 1/3 (h), in supernatants was measured by <t>ELISA.</t> Data from three independent experiments with different donors; symbols show the mean of duplicate wells for each individual donor. Differences between multiple groups were analysed using one-way analysis of variance (ANOVA) with Tukey's post hoc test (horizontal bars and error bars are means + s .e.m. of the three donors). P-values from Tukey tests, where significant are shown on the graphs. NS = ANOVA not significant.
Ifn α Human Verikinetm Elisa Kit, supplied by PBL Assay, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ifn-α human verikinetm elisa kit/product/PBL Assay
Average 90 stars, based on 1 article reviews
ifn-α human verikinetm elisa kit - by Bioz Stars, 2026-05
90/100 stars
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human interferon alpha 21 (ifna21) elisa kit  (Bioss)
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human interferon alpha-21(ifna21) elisa kit  (Cusabio)
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Human Interferon alpha-21(IFNA21) ELISA kit
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IFN-α, -β, -λ gene expression and protein release in MDM/TNF/IFNg, MDM/IL4 and MDM. MDM/TNF/IFNg, MDM/IL4 and MDM were treated with infectious RV16 or media alone and cells and supernatants were harvested at 8 h and 24 h or 72 h as indicated. Cell mRNA expression of IFN-α subtypes detected by primers pairs IFN-α.1 and IFN-α.2, panels (a) and (b) and mRNA expression of IFNB (c), IFN λ2/3 (d) and IFN λ1 (e) were measured by RT-PCR. Release of IFN-α (f), -β (g), -λ 1/3 (h), in supernatants was measured by ELISA. Data from three independent experiments with different donors; symbols show the mean of duplicate wells for each individual donor. Differences between multiple groups were analysed using one-way analysis of variance (ANOVA) with Tukey's post hoc test (horizontal bars and error bars are means + s .e.m. of the three donors). P-values from Tukey tests, where significant are shown on the graphs. NS = ANOVA not significant.

Journal: EBioMedicine

Article Title: M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations

doi: 10.1016/j.ebiom.2020.102734

Figure Lengend Snippet: IFN-α, -β, -λ gene expression and protein release in MDM/TNF/IFNg, MDM/IL4 and MDM. MDM/TNF/IFNg, MDM/IL4 and MDM were treated with infectious RV16 or media alone and cells and supernatants were harvested at 8 h and 24 h or 72 h as indicated. Cell mRNA expression of IFN-α subtypes detected by primers pairs IFN-α.1 and IFN-α.2, panels (a) and (b) and mRNA expression of IFNB (c), IFN λ2/3 (d) and IFN λ1 (e) were measured by RT-PCR. Release of IFN-α (f), -β (g), -λ 1/3 (h), in supernatants was measured by ELISA. Data from three independent experiments with different donors; symbols show the mean of duplicate wells for each individual donor. Differences between multiple groups were analysed using one-way analysis of variance (ANOVA) with Tukey's post hoc test (horizontal bars and error bars are means + s .e.m. of the three donors). P-values from Tukey tests, where significant are shown on the graphs. NS = ANOVA not significant.

Article Snippet: IFN-α, IFN-β, IFN-λ1/3, CCL22/MDC, CCL17/TARC, CCL18/PARC and IL-10 proteins were quantified by enzyme-linked immunosorbent assay (ELISA) in supernatants collected and stored at −80 °C using commercially available paired antibodies and standards, following the manufacturer's instructions using the IFN-α human VeriKineTM ELISA kit (PBL Assay Science Inc.) detecting all human IFN-α subtypes except IFN-αF encoded by gene IFNA21 , human IFN-β ELISA kit (FUJIREBIO INC.), human IL-10 ELISA Ready-Set-Go! (eBioscience), human CCL22/MDC, human IL-29/IL-28B (IFN-λ1/3), human CCL17/TARC and human CCL18/PARC DuoSets ELISA kits (R&D Systems).

Techniques: Gene Expression, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

IL-10, MDC/CCL22 and TARC/CCL17 protein release from MDM/TNF/IFNg, MDM/IL4 and MDM with or without RV16 infection. MDM/TNF/IFNg, MDM/IL4 and MDM were infected with RV16 or treated with media alone and supernatants were harvested at the indicated time points. Release of IL-10 (a), MDC (b) and TARC (c) in supernatants was measured by ELISA. Data from three independent experiments with different donors; symbols show the mean of duplicate wells for each individual donor. Differences between multiple groups were analysed using one-way analysis of variance (ANOVA) with Tukey's post hoc test (horizontal bars and error bars are means + s.e.m. of the three donors). P-values from Tukey tests, where significant are shown on the graphs. NS = ANOVA not significant.

Journal: EBioMedicine

Article Title: M1-like macrophages are potent producers of anti-viral interferons and M1-associated marker-positive lung macrophages are decreased during rhinovirus-induced asthma exacerbations

doi: 10.1016/j.ebiom.2020.102734

Figure Lengend Snippet: IL-10, MDC/CCL22 and TARC/CCL17 protein release from MDM/TNF/IFNg, MDM/IL4 and MDM with or without RV16 infection. MDM/TNF/IFNg, MDM/IL4 and MDM were infected with RV16 or treated with media alone and supernatants were harvested at the indicated time points. Release of IL-10 (a), MDC (b) and TARC (c) in supernatants was measured by ELISA. Data from three independent experiments with different donors; symbols show the mean of duplicate wells for each individual donor. Differences between multiple groups were analysed using one-way analysis of variance (ANOVA) with Tukey's post hoc test (horizontal bars and error bars are means + s.e.m. of the three donors). P-values from Tukey tests, where significant are shown on the graphs. NS = ANOVA not significant.

Article Snippet: IFN-α, IFN-β, IFN-λ1/3, CCL22/MDC, CCL17/TARC, CCL18/PARC and IL-10 proteins were quantified by enzyme-linked immunosorbent assay (ELISA) in supernatants collected and stored at −80 °C using commercially available paired antibodies and standards, following the manufacturer's instructions using the IFN-α human VeriKineTM ELISA kit (PBL Assay Science Inc.) detecting all human IFN-α subtypes except IFN-αF encoded by gene IFNA21 , human IFN-β ELISA kit (FUJIREBIO INC.), human IL-10 ELISA Ready-Set-Go! (eBioscience), human CCL22/MDC, human IL-29/IL-28B (IFN-λ1/3), human CCL17/TARC and human CCL18/PARC DuoSets ELISA kits (R&D Systems).

Techniques: Infection, Enzyme-linked Immunosorbent Assay